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The cholinergic neurons within the PPN and LDT regulate both of those SNc and VTA neurons through activating acetylcholine receptors in these neurons [eight]. Combining optogenetic and brain slice patch-clamp tactics, Xiao et al. [8] shown that 5–10?s of optogenetic stimulation of cholinergic projections from your PPN and LDT evokes inward curren

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